Difference between revisions of "Part:BBa K4814000"
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Hha is a biofilm reducing protein which decreases initial biofilm formation (Hong, S. H., Lee, J., & Wood, T. K., 2010). Hha is a protein of the Hha family, and it is very similar to YmoA protein from Yersinia enterocolitica (Balsalobre, C., et al., 1996).  Evidence has shown that hha interacts with H-NS, a type of chromatin-associated protein, which then expresses virulence factors (Madrid, C., Nieto, J. M., & Juárez, A., 2001). By using this protein, the bacteria will kill itself. This part is used together with RecA (BBa_K629001) to form a composite part (BBa_K4814001).
 
Hha is a biofilm reducing protein which decreases initial biofilm formation (Hong, S. H., Lee, J., & Wood, T. K., 2010). Hha is a protein of the Hha family, and it is very similar to YmoA protein from Yersinia enterocolitica (Balsalobre, C., et al., 1996).  Evidence has shown that hha interacts with H-NS, a type of chromatin-associated protein, which then expresses virulence factors (Madrid, C., Nieto, J. M., & Juárez, A., 2001). By using this protein, the bacteria will kill itself. This part is used together with RecA (BBa_K629001) to form a composite part (BBa_K4814001).
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<td><img src=https://static.igem.wiki/teams/4814/wiki/lab/hha-uvb.png alt=""style="width:400px;height:auto;">
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<img src=https://static.igem.wiki/teams/4814/wiki/lab/hha-h2o2.png alt=""style="width:400px;height:auto;">
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<p>Figure 1 & 2. The graph of the OD600 of RecA(K6)-hha after being treated with UVB (left) and H2O2 (right).</p>
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<img src=https://static.igem.wiki/teams/4814/wiki/lab/hha-na.png alt=""style="width:400px;height:auto;">
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<img src=https://static.igem.wiki/teams/4814/wiki/lab/hha-ap.png alt=""style="width:400px;height:auto;">
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<p>Figure 3 & 4. The graph of the OD600 of RecA(K6)-hha after being treated with Nalidixic acid (left) and Aspartame (right).</p>
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References:
 
References:

Revision as of 08:24, 9 October 2023

hha

Hha is a biofilm reducing protein which decreases initial biofilm formation (Hong, S. H., Lee, J., & Wood, T. K., 2010). Hha is a protein of the Hha family, and it is very similar to YmoA protein from Yersinia enterocolitica (Balsalobre, C., et al., 1996). Evidence has shown that hha interacts with H-NS, a type of chromatin-associated protein, which then expresses virulence factors (Madrid, C., Nieto, J. M., & Juárez, A., 2001). By using this protein, the bacteria will kill itself. This part is used together with RecA (BBa_K629001) to form a composite part (BBa_K4814001).

Figure 1 & 2. The graph of the OD600 of RecA(K6)-hha after being treated with UVB (left) and H2O2 (right).

Figure 3 & 4. The graph of the OD600 of RecA(K6)-hha after being treated with Nalidixic acid (left) and Aspartame (right).


References:

Balsalobre, C., Juárez, A., Madrid, C., Mouriño, M., Prenafeta, A., & Muñoa, F. J. (1996). Complementation of the hha mutation in Escherichia coli by the ymoA gene from Yersinia enterocolitica: dependence on the gene dosage. Microbiology (Reading, England), 142 ( Pt 7), 1841–1846. https://doi.org/10.1099/13500872-142-7-1841

Hong, S. H., Lee, J., & Wood, T. K. (2010). Engineering global regulator Hha of Escherichia coli to control biofilm dispersal. Microbial biotechnology, 3(6), 717–728. https://doi.org/10.1111/j.1751-7915.2010.00220.x

Madrid, C., Nieto, J. M., & Juárez, A. (2001). Role of the Hha/YmoA family of proteins in the thermoregulation of the expression of virulence factors. International Journal of Medical Microbiology, 291(6-7), 425-432. https://doi.org/10.1078/1438-4221-00149


Sequence derived from: https://www.uniprot.org/uniprotkb/P0ACE6/entry

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]