Difference between revisions of "Part:BBa K4765015"
(→Introduction) |
Siliang Zhan (Talk | contribs) (→Characterization) |
||
| Line 11: | Line 11: | ||
We heterologously expressed codon-optimized AnAFP in ''E.coli'', endowing it with antifreeze capability. | We heterologously expressed codon-optimized AnAFP in ''E.coli'', endowing it with antifreeze capability. | ||
===Characterization=== | ===Characterization=== | ||
| + | ====Sequencing map==== | ||
| + | {| | ||
| + | | <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/anafp-sequence.png" alt="contributed by Fudan iGEM 2023"></html> | ||
| + | |- | ||
| + | | '''Figure1 Sequencing map of AnAFP''' | ||
| + | Sequencing was performed using the two primers：T7: 5-TAATACGACTCACTATAGG-3 and Kan-F: 5-ATTCTCACCGGATTCAGT-3. | ||
| + | |||
| + | |} | ||
<!-- --> | <!-- --> | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| Line 20: | Line 28: | ||
<partinfo>BBa_K4765015 parameters</partinfo> | <partinfo>BBa_K4765015 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
| + | |||
==References== | ==References== | ||
<references /> | <references /> | ||
Revision as of 07:12, 9 October 2023
AnAFP
Introduction
Ammopiptanthus nanus antifreeze protein (AnAFP) is an antifreeze protein derived from Ammopiptanthus nanus. It protects the stability of membrane structures under cold conditions by binding to or dissociating from the membrane. It prevents the coagulation of macromolecules and plays a regulatory role by binding to or dissociating from calcium ions[1].
Usage and Biology
We heterologously expressed codon-optimized AnAFP in E.coli, endowing it with antifreeze capability.
Characterization
Sequencing map
|
| Figure1 Sequencing map of AnAFP
Sequencing was performed using the two primers：T7: 5-TAATACGACTCACTATAGG-3 and Kan-F: 5-ATTCTCACCGGATTCAGT-3. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 451
References
- ↑ Deng, L.-Q., Yu, H.-Q., Liu, Y.-P., Jiao, P.-P., Zhou, S.-F., Zhang, S.-Z., Li, W.-C., & Fu, F.-L. (2014). Heterologous expression of antifreeze protein gene AnAFP from Ammopiptanthus nanus enhances cold tolerance in Escherichia coli and tobacco. Gene, 539(1), 132-140.https://doi.org/10.1016/j.gene.2014.01.013