Difference between revisions of "Part:BBa K4613015"

Revision as of 06:36, 9 October 2023

T3-YFP

T3 (BBa_K4613011) and C3 (BBa_K4613012) can form protein complexes by elastin-like polypeptides(ELPs) monomers containing SpyTags and SpyCatchers. Different functional proteins can be incorporated into the polymeric scaffolds in a flexible manner due to its programmability. In this part, NAU-CHINA 2023 incorporated Yellow Fluorescent Protein(YFP). We fused YFP into T3 to immobilize the fluorescent protein and test the combination between T3 and C3.

Reference

Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nat Commun,2021, 12 (1): 3422.
Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7.
Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 940
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1589
Illegal SapI site found at 8

@@ Line 5: / Line 5: @@
 T3 (BBa_K4613011) and C3 (BBa_K4613012) can form protein complexes by elastin-like polypeptides(ELPs) monomers containing SpyTags and SpyCatchers.
 Different functional proteins can be incorporated into the polymeric scaffolds in a flexible manner due to its programmability. In this part, NAU-CHINA 2023 incorporated Yellow Fluorescent Protein(YFP). We fused YFP into T3 to immobilize the fluorescent protein and test the combination between T3 and C3.
+==== Reference ====
+#Dai Z, Yang X, Wu F, et al.Living fabrication of functional semi-interpenetrating polymeric materials[J].Nat Commun,2021, 12 (1): 3422.
+#Zakeri B, Fierer J O, Celik E, et al.Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J].Proc Natl Acad Sci U S A,2012, 109 (12): E690-7.
+#Reddington S C, Howarth M.Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher[J].Curr Opin Chem Biol,2015, 29: 94-9.
 <!-- Add more about the biology of this part here