Difference between revisions of "Part:BBa K4743002"
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<center>Figure 1. The length of NadA.</center>
 
<center>Figure 1. The length of NadA.</center>
  
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<span class='h3bb'><big>'''Overall metabolic pathway of NMN'''</big></span>
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NadA is involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.
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<html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/nmn-metabolic-pathway.png" </html>
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<center>Figure 2. The Matabolic pathway of NMN production.</center>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 06:24, 9 October 2023


NadA+ADH1 terminator with adapter

NadA gene codes for quinolinate synthase, which turns iminoaspartate into quinolate. The whole pathway is shown below:


NAD biosynthesis, iminoaspartate => quinolinate => NAD

Length checking of nadA

We use electrophoresis to check the length of the fragment

Figure 1. The length of NadA.

Overall metabolic pathway of NMN

NadA is involved in the pathway of Astaxanthin production in PTSH-Taiwan's project.

Figure 2. The Matabolic pathway of NMN production.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1284
    Illegal BamHI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
    Illegal AgeI site found at 1297
  • 1000
    COMPATIBLE WITH RFC[1000]