Difference between revisions of "Part:BBa K4743005:Design"

(→‎Design Notes)
(→‎Design Notes)
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1. We choose the NAMPT from Ralstonia solanacearum, because it has the highest activity compare to other organism's NAMPT.
 
1. We choose the NAMPT from Ralstonia solanacearum, because it has the highest activity compare to other organism's NAMPT.
  
−
2.AAGGTACCGCAGGTGGGATCC is the forward primer of Adapter 1
+
2. AAGGTACCGCAGGTGGGATCC is the forward primer of Adapter 1
  
 
TCACCGGTGCAGGTGGAATTC is the reverse primer of Adapter 2
 
TCACCGGTGCAGGTGGAATTC is the reverse primer of Adapter 2

Revision as of 05:19, 9 October 2023


NadV+ADH1 terminator with adapter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1689
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1689
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1689
    Illegal BglII site found at 515
    Illegal BamHI site found at 16
    Illegal BamHI site found at 367
    Illegal BamHI site found at 975
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1689
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1689
    Illegal AgeI site found at 1702
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. We choose the NAMPT from Ralstonia solanacearum, because it has the highest activity compare to other organism's NAMPT.

2. AAGGTACCGCAGGTGGGATCC is the forward primer of Adapter 1

TCACCGGTGCAGGTGGAATTC is the reverse primer of Adapter 2

Also, BamHI is included in adapter 1 and EcoRI is included in Adpater 2. These two site can be used for cloning

Source

Ralstonia solanacearum

References