Difference between revisions of "Part:BBa K4645002"
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<partinfo>BBa_K4645002 short</partinfo> | <partinfo>BBa_K4645002 short</partinfo> | ||
− | Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies. Among the resulting polyclonal antibodies, a monoclonal antibody named REGN1909 was found to be the most effective in blocking the immune response to the recombinant dimeric Fel D1. Through a literature review, | + | Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies. Among the resulting polyclonal antibodies, a monoclonal antibody named REGN1909 was found to be the most effective in blocking the immune response to the recombinant dimeric Fel D1. Through a literature review, we discovered both light chain and heavy chain genes of REGN1909. By fusing the C terminal of the light chain with the N terminal of the heavy chain using a rigid linker (GGGGS)3, an scFv was obtained. An OmpA signal peptide was fused to the N terminal of the scFv to secrete it into the periplasmic space, where it can more effectively neutralize the antigen. |
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+ | ===Protein Molecular Structures=== | ||
+ | ===Design=== | ||
+ | ===Materials and Method=== | ||
+ | ===Result=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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<partinfo>BBa_K4645002 parameters</partinfo> | <partinfo>BBa_K4645002 parameters</partinfo> | ||
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Revision as of 04:56, 9 October 2023
NeuB: scFv ( single-chain fragment variable)that inhibits the immune response to the feline
Velocimmune mice were immunized with recombinant dimeric Fel D1 and produced polyclonal antibodies. Among the resulting polyclonal antibodies, a monoclonal antibody named REGN1909 was found to be the most effective in blocking the immune response to the recombinant dimeric Fel D1. Through a literature review, we discovered both light chain and heavy chain genes of REGN1909. By fusing the C terminal of the light chain with the N terminal of the heavy chain using a rigid linker (GGGGS)3, an scFv was obtained. An OmpA signal peptide was fused to the N terminal of the scFv to secrete it into the periplasmic space, where it can more effectively neutralize the antigen.
Protein Molecular Structures
Design
Materials and Method
Result
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 679
Illegal SpeI site found at 75 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 679
Illegal SpeI site found at 75 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 679
Illegal XhoI site found at 276 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 679
Illegal SpeI site found at 75 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 679
Illegal SpeI site found at 75 - 1000COMPATIBLE WITH RFC[1000]