Difference between revisions of "Part:BBa K4585003"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K4585003 short</partinfo> | <partinfo>BBa_K4585003 short</partinfo> | ||
− | It belongs to the N-terminal domain of zinc finger protein and binds to some DNA binding domains to inhibit transcription. | + | It belongs to the N-terminal domain of zinc finger protein and binds to some DNA binding domains to inhibit transcription. The KRAB domain is a protein-protein interaction domain which can bind to a variety of synergistic transcriptional inhibitors and transcription factors, so that KRAB zinc finger proteins as transcriptional factors or transcriptional regulators plays a transcriptional inhibitory function dependently on DNA binding. |
+ | <html> | ||
+ | |||
+ | <head> | ||
+ | |||
+ | </head> | ||
+ | |||
+ | <body> | ||
+ | <h2 class="pageContent-main__title"> | ||
+ | <!--put tile here, <h2>title</h2>, class="pageContent-main__title" means it is the main title--> | ||
+ | |||
+ | 1.Diagrams | ||
+ | </h2> | ||
+ | <div class="pageContent-main__textBox"> | ||
+ | <!--all the content must included in this div--> | ||
+ | <p style="text-align: center;"> | ||
+ | <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/krab.png"></p> | ||
+ | <!--put image's url here--> | ||
+ | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of KRAB</p> | ||
+ | |||
+ | |||
+ | </h2> | ||
+ | <div class="pageContent-main__textBox"> | ||
+ | <!--all the content must included in this div--> | ||
+ | <p style="text-align: center;"> | ||
+ | <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/krabdian.png"></p> | ||
+ | <!--put image's url here--> | ||
+ | <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Agarose gel electrophoresis map of KRAB fragment PCR product</p> | ||
+ | </div> | ||
+ | <h2 class="pageContent-main__title pageContent-main__subtitle"> | ||
+ | <!--class="pageContent-main__title" means it is the sub title--> | ||
+ | |||
+ | |||
+ | 2.Caution | ||
+ | </h2> | ||
+ | <div class="pageContent-main__textBox"> | ||
+ | <!--all the content must included in this div--> | ||
+ | <p>Different KRAB domains have different silencing effects on target genes, regardless of cell types. The KRAB protein domain must be linked to the DNA binding domains to play an inhibitory role. | ||
+ | </p> | ||
+ | </div> | ||
+ | <h2 class="pageContent-main__title pageContent-main__subtitle"> | ||
+ | <!--class="pageContent-main__title" means it is the sub title--> | ||
+ | |||
+ | </body> | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 02:12, 9 October 2023
KRAB
It belongs to the N-terminal domain of zinc finger protein and binds to some DNA binding domains to inhibit transcription. The KRAB domain is a protein-protein interaction domain which can bind to a variety of synergistic transcriptional inhibitors and transcription factors, so that KRAB zinc finger proteins as transcriptional factors or transcriptional regulators plays a transcriptional inhibitory function dependently on DNA binding.
1.Diagrams
Fig.1 The model diagram of KRAB
Fig.2 Agarose gel electrophoresis map of KRAB fragment PCR product
2.Caution
Different KRAB domains have different silencing effects on target genes, regardless of cell types. The KRAB protein domain must be linked to the DNA binding domains to play an inhibitory role.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 178