Difference between revisions of "Part:BBa K4585002"

 
 
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<partinfo>BBa_K4585002 short</partinfo>
 
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VP64 is a kind of protein that can activate downstream gene expression.
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VP64 is a kind of protein that can activate downstream gene expression. It is a transcription activation factor which can realize the activation of downstream UAS transcription.
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                1.Diagrams
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/vp64moshit.png"></p>
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1  The model diagram of VP64</p>
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                    <img width="400px" src="https://static.igem.wiki/teams/4585/wiki/gal4-vp64.png
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.2 Agarose gel electrophoresis map of VP64 fragment PCR product</p>
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                2.Caution
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                <p>GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:04, 9 October 2023

VP64

VP64 is a kind of protein that can activate downstream gene expression. It is a transcription activation factor which can realize the activation of downstream UAS transcription.

1.Diagrams

Fig.1 The model diagram of VP64

Fig.2 Agarose gel electrophoresis map of VP64 fragment PCR product

2.Caution

GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 138
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 138
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 138
  • 1000
    COMPATIBLE WITH RFC[1000]