Difference between revisions of "Part:BBa K4593020"

Revision as of 21:50, 8 October 2023

S. aureus in vivo elimination apparatus for E. coli

Overview

This part contains 3 genetic circuits: an S. aureus quorum sensing (QS) detection system (BBa_I746101), an Endolysin combinative expression system (BBa_K4593019), and a self-lysing release system (BBa_I746104 and BBa_K4593001).

As the final intended product of our project's S. aureus elimination module, this device could constitutively express S. aureus targeting endolysins (a set of enzymes that specifically lyses S. aureus) and detect the presence of S. aureus QS signal. If the QS signal molecule is detected, the self-lysing enzyme composite (Spn1s_LysRZ) will be activated, and the engineered cell will lyse itself to release the endolysin that eliminates pathogenic S. aureus.

Endolysin expression circuit

This circuit could constitutively express 3 types of S. aureus targeting endolysin-LysDZ25, ClyC, and Lys GH15-that have maximum efficiency conditions complementary with each other. This ensures that at least one type of endolysin will be functional under various conditions in the human digestive tract. The lytic efficiency of the endolysins with respect to concentration is being characterized separately in our project. For detailed information, see the following basic parts:

LysDZ25: BBa_K4593000

ClyC: BBa_K4593002

LysGH25: BBa_K4593003

QS-detection circuit

Release circuit

Sequence and Features