Difference between revisions of "Part:BBa K4591009"

Revision as of 16:58, 8 October 2023

T500-RFP-lox66-Hpall-lox71-XylSmut-tetO-sfGFP-T500

The plasmid is mainly used to detect and report the presence of PA and TPA, and visually monitor the expression through the GFP and RFP.During the strain is put into use, we can regulate the flipping sequence to detect the presence of TPA and activate the expression of GFP.In other situations, flipping expresses the RFP and plays an alternative role.Considering that in future product applications, the strain may not necessarily be used to degrade PET immediately after delivery, we designed the flipping system to cope with different use situations. For example, when applied in a factory, in order to reduce adverse effects during fermentation production, the engineered bacteria express antibacterial products before turning over, and then turn over to express downstream degradation modules until they are put into use. Or in the field, the strain produces substances that increase fertility into the soil before the sequence is flipped. In turn, the engineered bacteria could have more purposes.