Difference between revisions of "Part:BBa K4743002"
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NAD biosynthesis, iminoaspartate => quinolinate => NAD
 
NAD biosynthesis, iminoaspartate => quinolinate => NAD
  
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<span class='h3bb'><big>'''Length checking of nadA'''</big></span>
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We use electrophoresis to check the length of the fragment
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<html><img style="width:800px" src="https://static.igem.wiki/teams/4743/wiki/folder-for-parts/nada-nadb-length.png" </html>
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<center>Figure 1. The length of NadA.</center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:50, 8 October 2023


NadA+ADH1 terminator with adapter

NadA gene codes for quinolinate synthase, which turns iminoaspartate into quinolate. The whole pathway is shown below:


NAD biosynthesis, iminoaspartate => quinolinate => NAD

Length checking of nadA

We use electrophoresis to check the length of the fragment

Figure 1. The length of NadA.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1284
    Illegal BamHI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1284
    Illegal SpeI site found at 1089
    Illegal AgeI site found at 1297
  • 1000
    COMPATIBLE WITH RFC[1000]