Difference between revisions of "Part:BBa K200026"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K200026 short</partinfo> | <partinfo>BBa_K200026 short</partinfo> | ||
− | + | This intermediate construct contains a GFP reporter to and can be used to assess the activity of the RcsB receiver protein. Different promoters can be attached upstream of the RBS to complete this transcriptional unit. | |
+ | |||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Imperial iGEM2009 developed this part from [https://parts.igem.org/Part:BBa_K200000 BBa_K200000] and [https://parts.igem.org/Part:BBa_I13504 BBa_I13504] as part of [http://2009.igem.org/Team:Imperial_College_London <i>The E.ncapsulator</i>] project. | ||
− | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K200026 SequenceAndFeatures</partinfo> | <partinfo>BBa_K200026 SequenceAndFeatures</partinfo> |
Latest revision as of 22:22, 18 October 2009
RcsB+RBS+GFP+TT
This intermediate construct contains a GFP reporter to and can be used to assess the activity of the RcsB receiver protein. Different promoters can be attached upstream of the RBS to complete this transcriptional unit.
Usage and Biology
Imperial iGEM2009 developed this part from BBa_K200000 and BBa_I13504 as part of [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] project.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1324