Difference between revisions of "Part:BBa K4897007"

Revision as of 10:39, 8 October 2023

Capsular antigen fragment 1-Antimicrobial Peptides

What is it?

Caf1-AMP is a protein to captures and suppresses P. acne, our identified main pathogen for acne formation. In short, Caf1-AMP, after entering the pores on human faces at a relatively warm temperature, can cool down to repolymerize into a net-like structure which limits the movement of P. acne. The antimicrobial peptides attached to the protein can selectively kill the bacteria by disrupting the cell membrane. This synthetic protein contains two parts: Capsular antigen fragment 1 (Caf1) and Antimicrobial Peptides (AMPs). Caf1 is a bacterial protein that possesses three valuable features that benefit our project. First, it is a unique protein that is thermally reformable [1]. At high temperature, long polymers of Caf1 dissociate into subunits; at low temperature, the subunits reforms into polymers without losing their function. This feature allows the protein to enter tiny pores on human faces at warm temperatures and cool down into a net-like structure after minutes. Second, the protein is safe for human cells, as proved by previous research on its ability to encapsulate human dermal fibroblast cells while maintaining the cells’ lives [1]. Third, certain features could be added to Caf1 by attaching protein or peptides to the protein. This feature enables us to adopt AMP on the protein to effectively and selectively kill P. acne. Antimicrobial Peptides (AMPs) are small peptide molecules that naturally exist for the immune system of animals [2]. In general, it adopts a broad range of mechanisms to defend the host against a variety of pathogens including bacteria. Through research, we found effective AMP that is selectively harmful for P. acne and use it in our synthetic protein. Overall, Caf1-AMP serves an important role in defending the skin from P. acne and preventing the formation of acne vulgaris.

Usage and Biology

Caf1-AMP is used to capture and suppress P. acne on human skin. While the Caf1 subunit of this part can limit the movement, specifically escape, of P. acne, the AMP subunit is able to damage the cell membrane of P. acne. Furthermore, AMP is effective for many pathogens, not only P. acne. Thus, Caf-AMP can be technically used to suppress other bacteria, but this is not recommended.

Characterization

Fig. 2. Characterization of Caf1-AMP using Western Blotting

Lane M is the Marker, and all other lanes are repeated results of Caf-AMP at 30 Celsius Degrees.

References

[1]Dura, G., Peters, D. L., Waller, H. L., Yemm, A. I., Perkins, N. J., Ferreira, A., … Fulton, D. (2020). A Thermally Reformable Protein Polymer. 6(11), 3132–3151. https://doi.org/10.1016/j.chempr.2020.09.020 [2]Zhang, Q.-Y., Yan, Z.-B., Meng, Y.-M., Hong, X.-Y., Shao, G., Ma, J.-J., … Fu, C.-Y. (2021). Antimicrobial peptides: mechanism of action, activity and clinical potential. Military Medical Research, 8, 48. https://doi.org/10.1186/s40779-021-00343-2.

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+Lane M is the Marker, and all other lanes are repeated results of Caf-AMP at 30 Celsius Degrees.
 ===References===
 [1]Dura, G., Peters, D. L., Waller, H. L., Yemm, A. I., Perkins, N. J., Ferreira, A., … Fulton, D. (2020). A Thermally Reformable Protein Polymer. 6(11), 3132–3151. https://doi.org/10.1016/j.chempr.2020.09.020
 [2]Zhang, Q.-Y., Yan, Z.-B., Meng, Y.-M., Hong, X.-Y., Shao, G., Ma, J.-J., … Fu, C.-Y. (2021). Antimicrobial peptides: mechanism of action, activity and clinical potential. Military Medical Research, 8, 48. https://doi.org/10.1186/s40779-021-00343-2.