Difference between revisions of "Part:BBa K4897000"

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===What is it?===
 
===What is it?===
  
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BS DNA-50 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. The binding region is the key element of binding the P. acne 16s rRNA gene. The DNA ligase will perform the ligation of the single-strand DNA meanwhile the phi29 will generate double-stranded DNA through amplification primers
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BS DNA-50 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. The binding region is the key element of binding the P. acne 16s rRNA gene [1]. The DNA ligase will perform the ligation of the single-strand DNA meanwhile the phi29 will generate double-stranded DNA through amplification primers
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===Usage and Biology===
 
===Usage and Biology===
  
 
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<img src="https://static.igem.wiki/teams/4897/wiki/parts/l-rca-mechanism.png" border="2" width="900" height="auto" />
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<table width="100%" border="10" cellspacing="0" cellpadding="0">
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  <tr>
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BS DNA-322 is the red line like a padlock. The black sequence represents the P. acne’s DNA specific to the binding. The red boxes represent the binding regions of the two ends of BS DNA50 (notice that the two ends are not connected). The purple boxes represent the random sequences generated. The Blue box represents the amplification region for RCA.
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    <td align="center"><img src="https://static.igem.wiki/teams/4897/wiki/parts/l-rca-mechanism.png" width="900" height="auto" /> </td>
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    <td align="center">Fig. 1. The process of BS DNA binding to P. acne DNA.</td>
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</table>
 
</html>
 
</html>
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Overall, theoretically, through ligation and rolling circle amplification (L-RCA), BS DNA-50 should have high selectivity for detecting the 16S rRNA gene of P. acne.
 
Overall, theoretically, through ligation and rolling circle amplification (L-RCA), BS DNA-50 should have high selectivity for detecting the 16S rRNA gene of P. acne.
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<partinfo>BBa_K4897000 parameters</partinfo>
 
<partinfo>BBa_K4897000 parameters</partinfo>
 
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===Reference===
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[1]Nakamura, M., Kametani, I., Higaki, S., & Yamagishi, T. (2003). Identification of Propionibacterium acnes by polymerase chain reaction for amplification of 16S ribosomal RNA and lipase genes. Anaerobe, 9(1), 5–10. https://doi.org/10.1016/s1075-9964(03)00061-1.

Latest revision as of 10:10, 8 October 2023


BS DNA-50 (BS DNA 1.0) using in L-RCA for detecting P. acne

What is it?

BS DNA-50 was designed by BS United China as a single-stranded DNA segment complementary to the 131 base pairs of the 16S rRNA gene of P. acne. The composition of the DNA has three categories: binding region (two ends), amplification region, and random region. The binding region is the key element of binding the P. acne 16s rRNA gene [1]. The DNA ligase will perform the ligation of the single-strand DNA meanwhile the phi29 will generate double-stranded DNA through amplification primers

Usage and Biology

Fig. 1. The process of BS DNA binding to P. acne DNA.

Overall, theoretically, through ligation and rolling circle amplification (L-RCA), BS DNA-50 should have high selectivity for detecting the 16S rRNA gene of P. acne.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 27
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 27
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 27
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

[1]Nakamura, M., Kametani, I., Higaki, S., & Yamagishi, T. (2003). Identification of Propionibacterium acnes by polymerase chain reaction for amplification of 16S ribosomal RNA and lipase genes. Anaerobe, 9(1), 5–10. https://doi.org/10.1016/s1075-9964(03)00061-1.