Difference between revisions of "Part:BBa K4767016:Design"
(→Design Notes) |
Airplan mod (Talk | contribs) (→Design Notes) |
||
| (4 intermediate revisions by 2 users not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | This is a combination of a strong RBS, a double terminator, followed by arsenic sensing and enhancer: <i>ars</i> promotor, RBS,<i>arsR</i> and luxR(△2-162). | + | This is a combination of a strong RBS, a double terminator, followed by arsenic sensing and enhancer: <i>ars</i> promotor, RBS, <i>arsR</i> and <i>luxR</i>(△2-162). |
===Source=== | ===Source=== | ||
| − | + | This part is from <i>Escherichia coli</i> and <i>Vibrio fischeri</i>,assembled by BBa_K4767001, BBa_J34801 , BBa_J15101 , BBa_B0015 and BBa_K4767000. | |
===References=== | ===References=== | ||
Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4. | Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4. | ||
| + | |||
Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1. | Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1. | ||
Latest revision as of 09:13, 8 October 2023
RBS-TT-Pars-RBS-arsR-luxR(△2-162)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 427
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a combination of a strong RBS, a double terminator, followed by arsenic sensing and enhancer: ars promotor, RBS, arsR and luxR(△2-162).
Source
This part is from Escherichia coli and Vibrio fischeri,assembled by BBa_K4767001, BBa_J34801 , BBa_J15101 , BBa_B0015 and BBa_K4767000.
References
Nistala, G.J., et al., A modular positive feedback-based gene amplifier. J Biol Eng, 2010. 4: p. 4.
Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.