Difference between revisions of "Part:BBa K4767014"
 
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<partinfo>BBa_K4767014 short</partinfo>
 
<partinfo>BBa_K4767014 short</partinfo>
  
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Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor <i>arsR</i> (BBa_J15101), and TT(BBa_B015). This part is an easy BioBrick.
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Uses a factor P<i><sub>ars</sub></i>(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor <i>arsR</i> (BBa_J15101), and TT(BBa_B0015). This part is an easy BioBrick.
  
  

Latest revision as of 08:32, 8 October 2023


Pars-RBS-arsR-TT

Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR (BBa_J15101), and TT(BBa_B0015). This part is an easy BioBrick.


Usage and Biology

This part comes from E. coli, which contains arsR (transcriptional regulator), arsB (arsenate permease), and arsC (arsenate reductase). When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the ars promoter and blocks transcription. Once arsenic is present, it binds to ArsR and changes the local structure of the promoter to activate the transcription of the ars genes and clear arsenic in the cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 270
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.