Difference between revisions of "Part:BBa K4767007"
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<partinfo>BBa_K4767007 short</partinfo> | <partinfo>BBa_K4767007 short</partinfo> | ||
| − | Uses a factor | + | Uses a factor P<sub><i>ars</i></sub>(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor <i>arsR</i>(BBa_J15101), c-type cytochromes <i>mtrC</i>(BBa_K3102020) and TT(BBa_B0015). This composite part is used in the iron reduction experiment and MFC. |
===Usage and Biology=== | ===Usage and Biology=== | ||
This part is a arsenic reporter with the reporter gene <i>mtrC</i>. MtrC is a c-type cytochromes from <i>Shewanella oneidensis</i>. It plays an important role in its extracellular electron transfer. To verify this part can respond to arsenic, we transfer this part into a <i>S. oneidensis</i> strain with the <i>mtrC</i> gene mutant. Then we cultured the strain with a arsenic concentration gradient. When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the <i>ars</i> promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the <i>mtrC</i> genes. As the result showed, strains with higher arsenic has a faster iron reduction rate, indicating the more expression of <i>mtrC</i>. | This part is a arsenic reporter with the reporter gene <i>mtrC</i>. MtrC is a c-type cytochromes from <i>Shewanella oneidensis</i>. It plays an important role in its extracellular electron transfer. To verify this part can respond to arsenic, we transfer this part into a <i>S. oneidensis</i> strain with the <i>mtrC</i> gene mutant. Then we cultured the strain with a arsenic concentration gradient. When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the <i>ars</i> promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the <i>mtrC</i> genes. As the result showed, strains with higher arsenic has a faster iron reduction rate, indicating the more expression of <i>mtrC</i>. | ||
| − | <center> | + | <center>https://static.igem.wiki/teams/4767/wiki/part/img-1170.png</center> |
<center>Fig. Iron reduction curves with different arsenic concentrations</center> | <center>Fig. Iron reduction curves with different arsenic concentrations</center> | ||
Latest revision as of 08:07, 8 October 2023
Pars-RBS-arsR-RBS-mtrC-TT
Uses a factor Pars(BBa_K4767001) ,strong RBS(BBa_J34801), DNA binding transcriptional repressor arsR(BBa_J15101), c-type cytochromes mtrC(BBa_K3102020) and TT(BBa_B0015). This composite part is used in the iron reduction experiment and MFC.
Usage and Biology
This part is a arsenic reporter with the reporter gene mtrC. MtrC is a c-type cytochromes from Shewanella oneidensis. It plays an important role in its extracellular electron transfer. To verify this part can respond to arsenic, we transfer this part into a S. oneidensis strain with the mtrC gene mutant. Then we cultured the strain with a arsenic concentration gradient. When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the ars promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the mtrC genes. As the result showed, strains with higher arsenic has a faster iron reduction rate, indicating the more expression of mtrC.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 270
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 751
References
Xiaoqiang Jia, Bu Rongrong, Zhao Tingting, et al. Sensitive and Specific Whole-Cell Biosensor for Arsenic Detection[J]. Applied and environmental microbiology, 2019, 85(11): 1.