Difference between revisions of "Part:BBa K4891005"
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===Usage and Biology===
 
===Usage and Biology===
  
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<h1>1 Plasmid Construction</h1>
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<h3>1 Plasmid Construction</h3>
 
Colony PCR results show that aroGopt, aroB, aroD, and aroE genes have been inserted into plasmid pTrcHisA (Fig 1).
 
Colony PCR results show that aroGopt, aroB, aroD, and aroE genes have been inserted into plasmid pTrcHisA (Fig 1).
 
<p style="text-align: center!important;">https://static.igem.wiki/teams/4891/wiki/parts/1.png</p>
 
<p style="text-align: center!important;">https://static.igem.wiki/teams/4891/wiki/parts/1.png</p>

Revision as of 07:43, 8 October 2023


aroGfbr-aroB-aroD-aroE

It is the key gene element for constructing shikimate biosynthetic pathway. Using this recombinant plasmid, shikimate could be generated from phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) by multi-enzyme cascade reaction.

Usage and Biology

1 Plasmid Construction

Colony PCR results show that aroGopt, aroB, aroD, and aroE genes have been inserted into plasmid pTrcHisA (Fig 1).

1.png

2.png

Fig 1. Construction of pTrcHisA-aroGfbr-aroB-aroD-aroE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2175
    Illegal XhoI site found at 934
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1965
    Illegal AgeI site found at 2509
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2362
    Illegal SapI.rc site found at 241