Difference between revisions of "Part:BBa K4891005"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | ===Characterization=== | |
− | + | '''2023 Hangzhou-SDG Team characterized this part as a suicide switch''' | |
+ | We tested the suicide switches based on the amounts of spore formation and the spore germination rates. Engineered strains were inoculated to the larvae of the greater wax moth (Galleria mellonella). The Petri dishes containing the pest corpses were placed under sunshine for days until spores formed outside the insect body. Spores were collected by vertexing and the concentrations of spore suspensions were checked under a microscope. | ||
+ | |||
<html> | <html> | ||
<body> | <body> | ||
<figure> | <figure> | ||
<div class = "center"> | <div class = "center"> | ||
− | <center>< img src = "https://static.igem.wiki/teams/4891/wiki/parts/1.png" style = "width: | + | <center>< img src = "https://static.igem.wiki/teams/4891/wiki/parts/1.png" style = "width:500px"></center> |
− | + | ||
</div> | </div> | ||
− | <figcaption><center> | + | <figcaption><center>Figure 1. A. The amount of spore formation by each strain on G. mellonella corpses; B. The spore germination rate of each strain in liquid PDB media after 10h. *: p < 0.01; **: p < 0.001. </center></figcaption> |
</figure> | </figure> | ||
</body> | </body> |
Revision as of 07:27, 8 October 2023
aroGfbr-aroB-aroD-aroE
It is the key gene element for constructing shikimate biosynthetic pathway. Using this recombinant plasmid, shikimate could be generated from phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) by multi-enzyme cascade reaction.
Usage and Biology
Characterization
2023 Hangzhou-SDG Team characterized this part as a suicide switch We tested the suicide switches based on the amounts of spore formation and the spore germination rates. Engineered strains were inoculated to the larvae of the greater wax moth (Galleria mellonella). The Petri dishes containing the pest corpses were placed under sunshine for days until spores formed outside the insect body. Spores were collected by vertexing and the concentrations of spore suspensions were checked under a microscope.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2175
Illegal XhoI site found at 934 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1965
Illegal AgeI site found at 2509 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2362
Illegal SapI.rc site found at 241