Difference between revisions of "Part:BBa K190017:Design"
m (→Design Notes) |
(→Source) |
||
(One intermediate revision by one other user not shown) | |||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | The | + | The CueR regulated promoter has been cloned into the [https://parts.igem.org/Part:BBa_J61002 BBa_J61002-R0040] plasmid for construction of promoter basic parts and their derivatives. |
Insertion of a promoter element between the XbaI and SpeI sites resulted in a RFP reporter while retaining the ability to do biobrick assembly. | Insertion of a promoter element between the XbaI and SpeI sites resulted in a RFP reporter while retaining the ability to do biobrick assembly. | ||
Line 15: | Line 15: | ||
===Source=== | ===Source=== | ||
− | E.coli TOP10 | + | ''E.coli'' TOP10 |
===References=== | ===References=== |
Latest revision as of 18:45, 18 October 2009
Copper Promoter (CueR regulated)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The CueR regulated promoter has been cloned into the BBa_J61002-R0040 plasmid for construction of promoter basic parts and their derivatives.
Insertion of a promoter element between the XbaI and SpeI sites resulted in a RFP reporter while retaining the ability to do biobrick assembly.
Source
E.coli TOP10