Difference between revisions of "Part:BBa K4765019"
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We heterologously expressed codon-optimized XRCC1 in ''E. coli'', endowing it with anti-UV capability. | We heterologously expressed codon-optimized XRCC1 in ''E. coli'', endowing it with anti-UV capability. | ||
===Characterization=== | ===Characterization=== | ||
| − | ==== | + | ====Sequencing map==== |
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| <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/sequencing-map-of-xrcc1.jpg" alt="contributed by Fudan iGEM 2023"></html> | | <html><img style="width:640px" src="https://static.igem.wiki/teams/4765/wiki/zsl/sequencing-map-of-xrcc1.jpg" alt="contributed by Fudan iGEM 2023"></html> | ||
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| − | | '''Figure1 | + | | '''Figure1 Sequencing map of XRCC11''' Sequencing starts from the T7 terminator, with the primer 5-GCTAGTTATTGCTCAGCGG-3. |
|} | |} | ||
Revision as of 03:01, 8 October 2023
XRCC1
Introduction
XRCC1 is a vital protein in DNA repair, particularly for single-strand breaks caused by radiation and alkylating agents. It collaborates with DNA ligase III, polymerase β, and poly (ADP-ribose) polymerase in base excision repair. It might also play a role in meiosis-related DNA processes. Although XRCC1 lacks enzymatic activity, it acts as a scaffold for repair enzymes, aiding in single-strand break repair, base excision repair, and nucleotide excision repair[1]. XRCC1's structure includes three domains, enabling interactions with various repair proteins. Additionally, it is involved in error-prone microhomology-mediated end joining repair of double-strand breaks, often leading to mutation-inducing deletions.
Usage and Biology
We heterologously expressed codon-optimized XRCC1 in E. coli, endowing it with anti-UV capability.
Characterization
Sequencing map
|
| Figure1 Sequencing map of XRCC11 Sequencing starts from the T7 terminator, with the primer 5-GCTAGTTATTGCTCAGCGG-3. |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 685
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1735
Reference
- ↑ London R. E. (2015). The structural basis of XRCC1-mediated DNA repair. DNA repair, 30, 90–103. https://doi.org/10.1016/j.dnarep.2015.02.005