Difference between revisions of "Part:BBa K4719020"
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<partinfo>BBa_K4719020 short</partinfo>
 
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4719020 SequenceAndFeatures</partinfo>
  
 
We fused a cellulose binding domain connected to a linker to the N-terminus of deacetylase ArCE4A to ensure a higher degree of deacetylation. For protein purification 6x his-tag was added to the N-terminus of cellulose binding domain.
 
We fused a cellulose binding domain connected to a linker to the N-terminus of deacetylase ArCE4A to ensure a higher degree of deacetylation. For protein purification 6x his-tag was added to the N-terminus of cellulose binding domain.
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<h3>Growth burden</h3>
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In order to work with <i>E. coli</i> for designing constructs and testing synthetic biology systems, the growth burden of said synthetic biology tools has to be measured. We performed growth burden evaluation by measuring OD600 for five hours of modified and unmodified <i>E. coli</i> DH5&alpha;. The composite of recombinant deacetylase CBD-ProThr box-ArCE4A did not inhibit the growth of <i>E. coli</i> as seen in Figure 1.
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<center><img src = "https://static.igem.wiki/teams/4719/wiki/partai/cbd-arce4a-growth-burden.png" style = "width:600px;"></center>
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<figcaption><center>Figure 1: growth burden of CBD-ProThr box-ArCE4A composite. </center></figcaption>
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4719020 SequenceAndFeatures</partinfo>
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Revision as of 22:32, 7 October 2023


CBD-ProThr box-ArCE4A chitin deacetylase and cellulose binding domain fusion protein
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 432
    Illegal NgoMIV site found at 961
    Illegal AgeI site found at 417
  • 1000
    COMPATIBLE WITH RFC[1000]

We fused a cellulose binding domain connected to a linker to the N-terminus of deacetylase ArCE4A to ensure a higher degree of deacetylation. For protein purification 6x his-tag was added to the N-terminus of cellulose binding domain.

Growth burden

In order to work with E. coli for designing constructs and testing synthetic biology systems, the growth burden of said synthetic biology tools has to be measured. We performed growth burden evaluation by measuring OD600 for five hours of modified and unmodified E. coli DH5α. The composite of recombinant deacetylase CBD-ProThr box-ArCE4A did not inhibit the growth of E. coli as seen in Figure 1.

Figure 1: growth burden of CBD-ProThr box-ArCE4A composite.