Difference between revisions of "Part:BBa K4579035"

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Following the part type standard of the Bee Toolkit created by Leonard et al., pBTK205 is a type 3 part that consists of the coding sequence for <i>GFP optim-1</i>, a green fluorescent protein.
 
Following the part type standard of the Bee Toolkit created by Leonard et al., pBTK205 is a type 3 part that consists of the coding sequence for <i>GFP optim-1</i>, a green fluorescent protein.
  
https://static.igem.wiki/teams/4579/wiki/btk-overhangs-low-ish-res.png
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<html><img src=https://static.igem.wiki/teams/4579/wiki/btk-overhangs-low-ish-res.png alt="CAPTIONNNNNN" style="width:800px;height:auto;"></html>
  
 
For additional info on the BTK standard, see: Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. <i>ACS Synthetic Biology, 7</i>(5), 1279-1290.
 
For additional info on the BTK standard, see: Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. <i>ACS Synthetic Biology, 7</i>(5), 1279-1290.

Revision as of 22:11, 7 October 2023


pBTK205 - GFP coding sequence

Following the part type standard of the Bee Toolkit created by Leonard et al., pBTK205 is a type 3 part that consists of the coding sequence for GFP optim-1, a green fluorescent protein.

CAPTIONNNNNN

For additional info on the BTK standard, see: Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. ACS Synthetic Biology, 7(5), 1279-1290.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13