Difference between revisions of "Part:BBa K4604006:Design"

 
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===Design Notes===
 
===Design Notes===
−
Change of start codon to adjust to E. coli. Additional site directed mutagenesis to result in a stop codon. No Methyonin triplet close to the inserted stop codon, translation should certainly end abruptly.   
+
Change of start codon to adjust to E. coli. Additional site directed mutagenesis to result in a stop codon. No Methyonine triplet close to the inserted stop codon, translation should certainly end abruptly.   
  
  

Revision as of 16:28, 7 October 2023


bluB mutated


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Change of start codon to adjust to E. coli. Additional site directed mutagenesis to result in a stop codon. No Methyonine triplet close to the inserted stop codon, translation should certainly end abruptly.



Source

Taken from Mycobacterium Smegmatis, synthesized by IDT.

References