Difference between revisions of "Part:BBa K4759035:Experience"
JianghuaChen (Talk | contribs) (→Applications of BBa_K4759035) |
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===Applications of BBa_K4759035=== | ===Applications of BBa_K4759035=== | ||
| − | + | we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags | |
(SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of | (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of | ||
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https://static.igem.wiki/teams/4759/wiki/protein-tags.png | https://static.igem.wiki/teams/4759/wiki/protein-tags.png | ||
| − | + | The application of protein tags | |
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 16:10, 7 October 2023
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K4759035
we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags
(SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of
Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.
The application of protein tags
User Reviews
UNIQa9c6294b9ea70081-partinfo-00000000-QINU UNIQa9c6294b9ea70081-partinfo-00000001-QINU