Difference between revisions of "Part:BBa K4759035:Experience"
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===Applications of BBa_K4759035=== | ===Applications of BBa_K4759035=== | ||
we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag. | we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag. | ||
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https://static.igem.wiki/teams/4759/wiki/protein-tags.png | https://static.igem.wiki/teams/4759/wiki/protein-tags.png | ||
Revision as of 16:09, 7 October 2023
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Applications of BBa_K4759035
we add a solubilization-promoting tag at the N-end of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.
The application of protein tags
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