Difference between revisions of "Part:BBa K4604017:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Assembled with Golden Gate.  
+
Assembled with Golden Gate. See more about exact sources on the pages of the BioBricks it is made up off. 
  
 
===References===
 
===References===

Revision as of 15:54, 7 October 2023


piG_02a (leaky_tetR_riboK12_mazF_mTurq)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
    Illegal AgeI site found at 1391
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2265


Design Notes

A GS-linker had to be cloned between MazF and mTurquoise to ensure correct folding and functionality of both proteins. A FLAG-tag was fused to MazF to make it detectable in western blot.


Source

Assembled with Golden Gate. See more about exact sources on the pages of the BioBricks it is made up off.

References