Difference between revisions of "Part:BBa K4727107"
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<partinfo>BBa_K4727107 short</partinfo> | <partinfo>BBa_K4727107 short</partinfo> | ||
| − | This guide DNA has been designed to work with dCas9 to target AMR related genes in Klebsiella pneumoniae | + | This guide DNA has been designed to work with dCas9 to target AMR related genes in <i>Klebsiella pneumoniae</i>. The guide binds 125bp downstream the TSS, on the RV strand of the gene. |
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| + | OmpA is a predominant outer membrane protein in <i>Enterobacteriaceae</i>. Its expression can be influenced by various environmental factors, among them the one exercised by glucose is the most discussed. In <i>Klebsiella pneumoniae</i>, KpOmpA mediates adhesion to a wide range of immune effector cells, facilitating respiratory tract and urinary infections. It stabilizes Gram-negative bacteria by anchoring their outer membrane to the peptidoglycan layer. | ||
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Revision as of 14:08, 7 October 2023
sgDNA for OmpA125 in K. pneumoniae
This guide DNA has been designed to work with dCas9 to target AMR related genes in Klebsiella pneumoniae. The guide binds 125bp downstream the TSS, on the RV strand of the gene.
OmpA is a predominant outer membrane protein in Enterobacteriaceae. Its expression can be influenced by various environmental factors, among them the one exercised by glucose is the most discussed. In Klebsiella pneumoniae, KpOmpA mediates adhesion to a wide range of immune effector cells, facilitating respiratory tract and urinary infections. It stabilizes Gram-negative bacteria by anchoring their outer membrane to the peptidoglycan layer.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]