Difference between revisions of "Part:BBa J15101:Experience"

Latest revision as of 09:39, 7 October 2023

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Applications of BBa_J15101

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 ===Applications of BBa_J15101===
-A relatively well-studied arsenic resistance operon is the one found in <i>Escherichia coli</i>, which contains <i>arsR</i> (transcriptional regulator), <i>arsB</i> (arsenite permease), and <i>arsC</i> (arsenate reductase). When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the ars promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the <i>ars</i> genes and clear arsenic in the cell. The <i>arsR</i> regulator and the promoter of this operon have been used to construct arsenic whole cell biosensors (WCB) in various microorganism hosts.
-When arsenic is absent, the transcription regulator ArsR binds to the ArsR-binding site (ABS) within the <i>ars</i> promoter and blocks transcription. Once arsenic is present, it binds to ArsR and activate the transcription of the downstream genes. We used <i>gfp</i> as a reporter to test its function of arsenic detection. Since this ars system comes from <i>E. coli</i> genome, to eliminate this impact, we used another model bacteria <i>Shewanella oneidensis</i> as the chassis cells to verify the function of this part. As the result shows below, with the arsenic concentration rises, the strain with the reporter produced higher fluorescence intensity.
-https://static.igem.wiki/teams/4767/wiki/part/bba-j15101.png
-Fig. Fluorescence curve of the reporter strain with different arsenic concentrations
 ===User Reviews===