Difference between revisions of "Part:BBa K200013"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K200013 short</partinfo>
 
<partinfo>BBa_K200013 short</partinfo>
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=Background=
  
 
Opiorphin is a pentapeptide (five amino acids long) that was isolated from human saliva in 1996. The amino acid sequence for opiorphin is Glutamine-Arginine-Phenylalanine-Serine-Arginine.
 
Opiorphin is a pentapeptide (five amino acids long) that was isolated from human saliva in 1996. The amino acid sequence for opiorphin is Glutamine-Arginine-Phenylalanine-Serine-Arginine.
  
 
Preliminary rat studies indicate that opiorphin has a pain killing effect six times stronger than that of morphine. With regards to its mechanism of action, opiorphin prevents the breakdown of enkephalins which are natural pain-killing opioids.  
 
Preliminary rat studies indicate that opiorphin has a pain killing effect six times stronger than that of morphine. With regards to its mechanism of action, opiorphin prevents the breakdown of enkephalins which are natural pain-killing opioids.  
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Since polypeptides can only be biologically synthesised if they start with the amino acid methionine, it has been necessary to design a cleavable peptide sequence starting with methionine. This sequence is found upstream of opiorphin and is enzymatically cleaved following incubation with the enzyme enterokinase.  Enterokinase is a highly specific serine protease that cleaves after the recognition sequence <i>Aspartic Acid - Aspartic Acid - Aspartic Acid - Aspartic Acid – Lysine</i>.
 
  
  

Revision as of 14:46, 18 October 2009

Opiorphin + EK cleavage site

Background

Opiorphin is a pentapeptide (five amino acids long) that was isolated from human saliva in 1996. The amino acid sequence for opiorphin is Glutamine-Arginine-Phenylalanine-Serine-Arginine.

Preliminary rat studies indicate that opiorphin has a pain killing effect six times stronger than that of morphine. With regards to its mechanism of action, opiorphin prevents the breakdown of enkephalins which are natural pain-killing opioids.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]