Difference between revisions of "Part:BBa J15101"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | Group:CUG_China | ||
| + | Author:Jian Liu | ||
| + | Summary:Once arsenic is present, it binds to ArsR and activate the transcription of the downstream genes. We used gfp as a reporter to test its function of arsenic detection. Since this ars system comes from E. coli genome, to eliminate this impact, we used another model bacteria Shewanella oneidensis as the chassis cells to verify the function of this part. | ||
| + | https://static.igem.wiki/teams/4767/wiki/part/bba-j15101-20231007161859.pdf | ||
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Revision as of 08:22, 7 October 2023
arsR coding sequence, Escherichia coli chromosomal ars repressor
Escherichia coli chromosomal arsR coding sequence, which encodes the ArsR repressor. This binds to the ars promoter and represses it in the absence of arsenate or arsenite (Cai, J., and DuBow, M.S. 1996. Expression of the Escherichia coli chromosomal ars operon. Canadian Journal of Microbiology 42, 662-671; Diorio, C., Cai, J., Marmor, J., Shinder, R., and DuBow, M.S. 1995. An Escherichia coli chromosomal ars operon homolog is functional in arsenic detoxification and is conserved in Gram negative bacteria. Journal of Bacteriology 177, 2050-2056).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 137
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]