Difference between revisions of "Part:BBa K4765115"
(→Usage and Biology) |
Siliang Zhan (Talk | contribs) (→Introduction) |
||
| Line 7: | Line 7: | ||
===Introduction=== | ===Introduction=== | ||
| − | This composite part is composed of FEN1 and ribozyme-assisted polycistronic co-expression system：pRAP. FEN1 plays a pivotal role in DNA maintenance, addressing 5' overhanging | + | This composite part is composed of FEN1 and ribozyme-assisted polycistronic co-expression system：pRAP. FEN1 plays a pivotal role in DNA maintenance, addressing 5' overhanging flaps formed when DNA strands can't bind correctly. |
| + | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
We constructed FEN1 into pRAP to endow ''E. coli'' with anti-UV capability. | We constructed FEN1 into pRAP to endow ''E. coli'' with anti-UV capability. | ||
Revision as of 02:39, 7 October 2023
Twister P1 + T7_RBS + FEN1 + stem-loop
Introduction
This composite part is composed of FEN1 and ribozyme-assisted polycistronic co-expression system：pRAP. FEN1 plays a pivotal role in DNA maintenance, addressing 5' overhanging flaps formed when DNA strands can't bind correctly.
Usage and Biology
We constructed FEN1 into pRAP to endow E. coli with anti-UV capability.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 617
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1195
Illegal AgeI site found at 301 - 1000COMPATIBLE WITH RFC[1000]