Difference between revisions of "Part:BBa K4724006"
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<partinfo>BBa_K4724006 short</partinfo> | <partinfo>BBa_K4724006 short</partinfo> | ||
− | We chose this site because that the active pocket amino acids are changed, altering the tertiary structure of the protein. | + | We inserted glutamate E between amino acids 93 and 94 of IsPETase.We chose this site because that the active pocket amino acids are changed, altering the tertiary structure of the protein. |
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Revision as of 16:02, 6 October 2023
IsPETaseS93_I94insE-6xHis Tag
We inserted glutamate E between amino acids 93 and 94 of IsPETase.We chose this site because that the active pocket amino acids are changed, altering the tertiary structure of the protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 56
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 56
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 793
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 56
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 56
Illegal AgeI site found at 549 - 1000COMPATIBLE WITH RFC[1000]