Difference between revisions of "Part:BBa K4800015"

 
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<partinfo>BBa_K4800015 short</partinfo>
 
<partinfo>BBa_K4800015 short</partinfo>
  
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The promoter is Ptrc, which can be expressed in Escherichia coli KA30. YciA-sfp-MmCAR is derived from BBa_K1655000. The carboxylic acid reductase MmCAR position 302 is mutated with acid-base, and glutamine is replaced with glutamic acid. Obtain YciA-sfp-MmCAR (Q302E), improve the efficiency of carboxylic acid reduction, and YahK is an aldehyde reductase. This fragment constructs the 5-hydroxyvaleric acid to 1,5-pentanediol pathway.
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GabT compiles 4-aminobutyrate aminotransferase GabT, which catalyzes the reaction: 5-aminopentanoate + 2-oxoglutarate ↔ L-glutamate + glutarate semialdehyde.
  
 
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Revision as of 14:27, 6 October 2023


GabT

GabT compiles 4-aminobutyrate aminotransferase GabT, which catalyzes the reaction: 5-aminopentanoate + 2-oxoglutarate ↔ L-glutamate + glutarate semialdehyde.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]