Difference between revisions of "Part:BBa K4665005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part results in a membrane fusion protein, meaning that extraction protocols for the protein should differ from normal extraction protocols | + | This composite part results in a membrane fusion protein, meaning that extraction protocols for the protein should differ from normal extraction protocols |
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===Source=== | ===Source=== | ||
Revision as of 13:52, 6 October 2023
SazCA-INPN Membrane Display Module
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 470
Illegal PstI site found at 592 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 470
Illegal PstI site found at 592 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 470
Illegal PstI site found at 592 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 470
Illegal PstI site found at 592
Illegal NgoMIV site found at 54
Illegal AgeI site found at 555 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This composite part results in a membrane fusion protein, meaning that extraction protocols for the protein should differ from normal extraction protocols
Source
SazCA is derived from Sulfurihydrogenibium azorense and INPN is derived from Pseudomonas syringae. Both sequences have been codon optimized for E. coli (BL21 DE3).
Our sequence for SazCa is derived from the paper by Zhu et al. (2022), which has been codon-optimized for E. coli BL21(DE3).
References
Zhu, Y., et al. (December 6, 2021). Surface display of carbonic anhydrase on Escherichia coli for CO2 capture and mineralisation. Synthetic and Systems biotechnology, 7(1): 460-473. https://doi.org/10.1016%2Fj.synbio.2021.11.008