Difference between revisions of "Part:BBa K2507000"
 
(10 intermediate revisions by 2 users not shown)
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K2507014 short</partinfo>
+
<partinfo>BBa_K2507000 short</partinfo>
  
 
== Background ==
 
== Background ==
Line 7: Line 7:
 
<body>
 
<body>
 
<p>
 
<p>
−
ThsS/R are two basic parts which belong to the two-component systems. This system is from marine Shewanella species. After validate this system in laboratory Escherichia coli, this system can function as a thiosulfate sensor and reporter. Because of thiosulfate is an indicator of gut inflammation, this system can be used as a sensor of gut inflammation. Combining with sfGFP or chromoprotein， after receive the sign from thsS, this system can finally repress the result by produce protein.
+
 
 +
ThsS (BBa_K2507000) and ThsR (BBa_K2507001), both codon-optimized for E. coli, are two basic parts which belong to the two-component system from the marine bacterium Shewanella halifaxensis. ThsS is the membrane-bound sensor kinase (SK) which can sense thiosulfate outside the cell, and ThsR is the DNA-binding response regulator(RR).
  
 
<p/>
 
<p/>
Line 21: Line 22:
 
<partinfo>BBa_K2507000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2507000 SequenceAndFeatures</partinfo>
  
−
 
+
==Reference==
 +
<p>
 +
Daeffler, K. N., Galley, J. D., Sheth, R. U., Ortiz‐Velez, L. C., Bibb, C. O., & Shroyer, N. F., et al. (2017). Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation. Molecular Systems Biology, 13(4), 923.
 +
</p>
 +
<p>
 +
Jackson MR, Melideo SL, Jorns MS (2012) Human sulfide: quinone oxidoreductase catalyzes the first step in hydrogen sulfide metabolism and produces a sulfane sulfur metabolite. Biochemistry 51: 6804 – 6815
 +
</p>
 +
<p>
 +
Levitt MD, Furne J, Springfield J, Suarez F, DeMaster E (1999) Detoxification of hydrogen sulfide and methanethiol in the cecal mucosa. J Clin Invest 104: 1107 – 1114
 +
</p>
 +
<p>
 +
Schmidl SR, Sheth RU, Wu A, Tabor JJ (2014) Refactoring and optimization of light-switchable Escherichia coli two-component systems. ACS Synth Biol 3: 820 – 831
 +
</p>
 +
<p>
 +
Vitvitsky V, Yadav PK, Kurthen A, Banerjee R (2015) Sulfide oxidation by a noncanonical pathway in red blood cells generates thiosulfate and polysulfides. J Biol Chem 290: 8310 – 8320
 +
</p>
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
Line 28: Line 44:
 
<html>
 
<html>
 
<br/>
 
<br/>
−
<h2 id="results">Results</h2>
+
 
 
<p>  </p>
 
<p>  </p>
  
Line 39: Line 55:
 
</p>
 
</p>
 
<br/><br/>
 
<br/><br/>
 +
 +
 
</body>
 
</body>
 
</html>
 
</html>

Latest revision as of 12:09, 6 October 2023

ThsS

Background

ThsS (BBa_K2507000) and ThsR (BBa_K2507001), both codon-optimized for E. coli, are two basic parts which belong to the two-component system from the marine bacterium Shewanella halifaxensis. ThsS is the membrane-bound sensor kinase (SK) which can sense thiosulfate outside the cell, and ThsR is the DNA-binding response regulator(RR).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1078
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 477
    Illegal BamHI site found at 525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1010

Reference

Daeffler, K. N., Galley, J. D., Sheth, R. U., Ortiz‐Velez, L. C., Bibb, C. O., & Shroyer, N. F., et al. (2017). Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation. Molecular Systems Biology, 13(4), 923.

Jackson MR, Melideo SL, Jorns MS (2012) Human sulfide: quinone oxidoreductase catalyzes the first step in hydrogen sulfide metabolism and produces a sulfane sulfur metabolite. Biochemistry 51: 6804 – 6815

Levitt MD, Furne J, Springfield J, Suarez F, DeMaster E (1999) Detoxification of hydrogen sulfide and methanethiol in the cecal mucosa. J Clin Invest 104: 1107 – 1114

Schmidl SR, Sheth RU, Wu A, Tabor JJ (2014) Refactoring and optimization of light-switchable Escherichia coli two-component systems. ACS Synth Biol 3: 820 – 831

Vitvitsky V, Yadav PK, Kurthen A, Banerjee R (2015) Sulfide oxidation by a noncanonical pathway in red blood cells generates thiosulfate and polysulfides. J Biol Chem 290: 8310 – 8320