Difference between revisions of "Part:BBa K4887001"
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The encoding gene of granule-bound starch synthase I (GBSSI) in sweet potato (Ipomoea batatas). It is one of the starch biosynthetic pathway genes on the starch biosynthetic pathway. | The encoding gene of granule-bound starch synthase I (GBSSI) in sweet potato (Ipomoea batatas). It is one of the starch biosynthetic pathway genes on the starch biosynthetic pathway. | ||
− | + | (1) GBSSI-sgRNA design | |
The selected sgRNA is located 70 bp downstream of the start codon, with a total sequence length of 27 bp (Fig. 1). sgRNA oligos were obtained by annealing with the designed primers following: | The selected sgRNA is located 70 bp downstream of the start codon, with a total sequence length of 27 bp (Fig. 1). sgRNA oligos were obtained by annealing with the designed primers following: | ||
Oligo 1 (GBSSI-sgRNA1): 5’-GTGGGGTTGGGTCAATTAGCCCTGAGGAGC-3' | Oligo 1 (GBSSI-sgRNA1): 5’-GTGGGGTTGGGTCAATTAGCCCTGAGGAGC-3' |
Revision as of 09:40, 6 October 2023
IbGBSSI
The encoding gene of granule-bound starch synthase I (GBSSI) in sweet potato (Ipomoea batatas). It is one of the starch biosynthetic pathway genes on the starch biosynthetic pathway. (1) GBSSI-sgRNA design The selected sgRNA is located 70 bp downstream of the start codon, with a total sequence length of 27 bp (Fig. 1). sgRNA oligos were obtained by annealing with the designed primers following: Oligo 1 (GBSSI-sgRNA1): 5’-GTGGGGTTGGGTCAATTAGCCCTGAGGAGC-3' Oligo 2 (GBSSI-sgRNA2): 5’-AACTGGTGGACTTGGAGATGTTCTTGGAGG-3’
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1459
Illegal BsaI.rc site found at 123
Illegal SapI site found at 1156
Illegal SapI.rc site found at 1225