Difference between revisions of "Part:BBa K4887001"
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The encoding gene of granule-bound starch synthase I (GBSSI) in sweet potato (Ipomoea batatas). It is one of the starch biosynthetic pathway genes on the starch biosynthetic pathway. | The encoding gene of granule-bound starch synthase I (GBSSI) in sweet potato (Ipomoea batatas). It is one of the starch biosynthetic pathway genes on the starch biosynthetic pathway. | ||
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+ | The selected sgRNA is located 70 bp downstream of the start codon, with a total sequence length of 27 bp (Fig. 1). sgRNA oligos were obtained by annealing with the designed primers following: | ||
+ | Oligo 1 (GBSSI-sgRNA1): 5’-GTGGGGTTGGGTCAATTAGCCCTGAGGAGC-3' | ||
+ | Oligo 2 (GBSSI-sgRNA2): 5’-AACTGGTGGACTTGGAGATGTTCTTGGAGG-3’ | ||
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Revision as of 09:39, 6 October 2023
IbGBSSI
The encoding gene of granule-bound starch synthase I (GBSSI) in sweet potato (Ipomoea batatas). It is one of the starch biosynthetic pathway genes on the starch biosynthetic pathway.
The selected sgRNA is located 70 bp downstream of the start codon, with a total sequence length of 27 bp (Fig. 1). sgRNA oligos were obtained by annealing with the designed primers following: Oligo 1 (GBSSI-sgRNA1): 5’-GTGGGGTTGGGTCAATTAGCCCTGAGGAGC-3' Oligo 2 (GBSSI-sgRNA2): 5’-AACTGGTGGACTTGGAGATGTTCTTGGAGG-3’
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1459
Illegal BsaI.rc site found at 123
Illegal SapI site found at 1156
Illegal SapI.rc site found at 1225