Difference between revisions of "Part:BBa K203108"

Revision as of 10:57, 18 October 2009

Plasma membrane targeting (Myrpalm)

Localization sequence to direct a protein into the cell membrane.

Usage and Biology

This localization signal is located at the N-terminal end of the amino acid chain. The myrpalm signalsequence causes a myristoylation and palmitoylation of the targeted protein. Both modifications lead to a binding to the cell membrane.[Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells. By: Zacharias, David A., Violin, Jonathan D., Newton, Alexandra C., Tsien, Roger Y., Science, 00368075, 5/3/2002, Vol. 296, Issue 5569].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 86
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 49
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 46

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K203108 short</partinfo>
-To be updated
-<!-- Add more about the biology of this part here
+Localization sequence to direct a protein into the cell membrane.
+[[Image:Myrpalm.jpg]]
 ===Usage and Biology===
+This localization signal is located at the N-terminal end of the amino acid chain. The myrpalm signalsequence causes a myristoylation and palmitoylation of the targeted protein. Both modifications lead to a binding to the cell membrane.[Partitioning of Lipid-Modified Monomeric GFPs into Membrane Microdomains of Live Cells. By: Zacharias, David A., Violin, Jonathan D., Newton, Alexandra C., Tsien, Roger Y., Science, 00368075, 5/3/2002, Vol. 296, Issue 5569].
-<!-- -->
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K203108 SequenceAndFeatures</partinfo>