Difference between revisions of "Part:BBa K4579034"

Revision as of 06:49, 6 October 2023

pBTK1001 part backbone

This is an entry vector from the Bee Microbiome Toolkit that is designed for initial part plasmid creation from PCR products or gBlocks. This plasmid and the PCR product are digested with BsmBI to form the initial part plasmid. Once they're in the pBTK1001 backbone, our parts are designed to be further digested with BsaI and integrated into an assembly vector such as pBTK1028 (BBa_K4579033). The first four bases and last four bases of the sequence for this entry vector are the overhangs generated after digestion with BsmBI. The entry vector plasmid contains a GFP dropout to allow for screening. This backbone was created by Peng Geng of the Barrick lab at the University of Texas at Austin.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4579034 short</partinfo>
-This is an entry vector from the Bee Microbiome Toolkit that is designed for initial part plasmid creation from PCR products or gBlocks. This plasmid and the PCR product are digested with BsmBI to form the initial part plasmid. Once they're in the pBTK1001 backbone, our parts are designed to be further digested with BsaI and integrated into an assembly vector such as pBTK1028 (BBa_K4579033). The first four bases and last four bases of the sequence for this entry vector are the overhangs generated after digestion with BsmBI. The entry vector plasmid contains a GFP dropout to allow for screening. This backbone was created by Peng Geng of the Barrick lab at the University of Texas at Austin.
+This is an entry vector from the Bee Microbiome Toolkit that is designed for initial part plasmid creation from PCR products or gBlocks. This plasmid and the PCR product are digested with BsmBI to form the initial part plasmid. Once they're in the pBTK1001 backbone, our parts are designed to be further digested with BsaI and integrated into an assembly vector such as pBTK1028 [https://parts.igem.org/Part:BBa_K4579033 (BBa_K4579033)]. The first four bases and last four bases of the sequence for this entry vector are the overhangs generated after digestion with BsmBI. The entry vector plasmid contains a GFP dropout to allow for screening. This backbone was created by Peng Geng of the Barrick lab at the University of Texas at Austin.
 <!-- Add more about the biology of this part here