Difference between revisions of "Part:BBa K4614002"
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<partinfo>BBa_K4614002 short</partinfo> | <partinfo>BBa_K4614002 short</partinfo> | ||
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We used T7 promoter to induce IPN and R5(part BBa_K461400) expression, IPN, a carrier protein from the genome of Pseudomonas syringae, used to display R5 on the surface of bacteria. | We used T7 promoter to induce IPN and R5(part BBa_K461400) expression, IPN, a carrier protein from the genome of Pseudomonas syringae, used to display R5 on the surface of bacteria. | ||
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In use, the modified E. coli can be cultured to the logarithmic stage, and IPTG with a final concentration of 1.0 ug/mL can be added to induce 3 h, and protein expression can be completed | In use, the modified E. coli can be cultured to the logarithmic stage, and IPTG with a final concentration of 1.0 ug/mL can be added to induce 3 h, and protein expression can be completed | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 06:43, 6 October 2023
Lnak-R5
We used T7 promoter to induce IPN and R5(part BBa_K461400) expression, IPN, a carrier protein from the genome of Pseudomonas syringae, used to display R5 on the surface of bacteria.
In use, the modified E. coli can be cultured to the logarithmic stage, and IPTG with a final concentration of 1.0 ug/mL can be added to induce 3 h, and protein expression can be completed
Sequence and Features