Difference between revisions of "Part:BBa K4579033"
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Following the part type standard of the Yeast and Bee Microbiome Toolkits, pBTK1028 is a type 56781 BsaI dropout vector that can be used to create first stage assemblies by combining with type 2, 3, and 4 parts according to the BTK/YTK standard. It can also be used as a BsmBI dropout vector for second stage assembly of parts begining with the ConLS overhang and ending with the ConRE overhang. This backbone was created by Shaunak Kar from the Ellington lab at The University of Texas at Austin. | Following the part type standard of the Yeast and Bee Microbiome Toolkits, pBTK1028 is a type 56781 BsaI dropout vector that can be used to create first stage assemblies by combining with type 2, 3, and 4 parts according to the BTK/YTK standard. It can also be used as a BsmBI dropout vector for second stage assembly of parts begining with the ConLS overhang and ending with the ConRE overhang. This backbone was created by Shaunak Kar from the Ellington lab at The University of Texas at Austin. | ||
| − | The first four bases of this part correspond to a type 5 part forward prefix, and the last four bases of this part correspond to a type 1 part reverse suffix. [[ | + | The first four bases of this part correspond to a type 5 part forward prefix, and the last four bases of this part correspond to a type 1 part reverse suffix. [[Image:<html><img src=https://static.igem.wiki/teams/4579/wiki/btk-overhangs-low-ish-res.png><html>|thumb|left|450px|Figure 1: ]] |
For literature describing the BTK and YTK standards, please see the References section for this part. | For literature describing the BTK and YTK standards, please see the References section for this part. | ||
Revision as of 05:08, 6 October 2023
pBTK1028 assembly backbone
Following the part type standard of the Yeast and Bee Microbiome Toolkits, pBTK1028 is a type 56781 BsaI dropout vector that can be used to create first stage assemblies by combining with type 2, 3, and 4 parts according to the BTK/YTK standard. It can also be used as a BsmBI dropout vector for second stage assembly of parts begining with the ConLS overhang and ending with the ConRE overhang. This backbone was created by Shaunak Kar from the Ellington lab at The University of Texas at Austin.
The first four bases of this part correspond to a type 5 part forward prefix, and the last four bases of this part correspond to a type 1 part reverse suffix. [[Image:
|thumb|left|450px|Figure 1: ]]
For literature describing the BTK and YTK standards, please see the References section for this part.
Sequence and Features