Difference between revisions of "Part:BBa K4883004"

 
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<partinfo>BBa_K4883004 short</partinfo>
 
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Insert a 2A self-cleaving peptide, PTV, between RIB1 and ADE4. PTV helps to achieve bicistronic gene expression in Saccharomyces cerevisiae. Use a strong constitutive promoter Ptef1 to overexpress RIB1 and ADE4.
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This is a complete expression cassette, constructed by inserting RIB1-PTV-ADE4 ([https://parts.igem.org/Part:BBa_K4883003 BBa_K4883003]) between a strong promoter Ptef1 and a CYC1 terminator.  
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===Usage and Biology===
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<partinfo>BBa_K4883004 parameters</partinfo>
 
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===Usage and Biology===

Revision as of 02:41, 6 October 2023


Ptef1-RIB1-PTV-ADE4-Tcyc1

This is a complete expression cassette, constructed by inserting RIB1-PTV-ADE4 (BBa_K4883003) between a strong promoter Ptef1 and a CYC1 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3298
    Illegal BamHI site found at 836
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 856
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 176



Usage and Biology