Difference between revisions of "Part:BBa K4579035"

Revision as of 23:29, 5 October 2023

pBTK205 - GFP coding sequence

Following the part type standard of the Bee Toolkit described by Leonard et al., pBTK205 is a type 3 part that consists of the coding sequence for GFPmut3, a green fluorescent protein.

The first four bases of this part correspond to a type 3 part forward prefix (TATG), and the last four bases of this part correspond to a type 3 part reverse suffix (GGAT).

For additional info on the BTK standard, see: Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. ACS Synthetic Biology, 7(5), 1279-1290.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 13

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4579035 short</partinfo>
-Following the part type standard of the Bee Toolkit, pBTK205 is a type 3 part that consists of the coding sequence for <i>GFPmut3</i>, a green fluorescent protein.
+Following the part type standard of the Bee Toolkit described by Leonard et al., pBTK205 is a type 3 part that consists of the coding sequence for <i>GFPmut3</i>, a green fluorescent protein.
 The first four bases of this part correspond to a type 3 part forward prefix (TATG), and the last four bases of this part correspond to a type 3 part reverse suffix (GGAT). https://static.igem.wiki/teams/4579/wiki/btk-overhangs-low-ish-res.png