Difference between revisions of "Part:BBa K4579033:Design"
Alexakmorton (Talk | contribs) (→Source) |
Alexakmorton (Talk | contribs) (→Design Notes) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | N/A - | + | N/A - This part was not designed by the UT iGEM team. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
This plasmid comes from the Bee Toolkit, as described in: Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. <i>ACS Synthetic Biology, 7</i>(5), 1279-1290. | This plasmid comes from the Bee Toolkit, as described in: Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. <i>ACS Synthetic Biology, 7</i>(5), 1279-1290. | ||
Revision as of 23:22, 5 October 2023
pBTK1028 assembly backbone
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal XbaI site found at 1229
Illegal XbaI site found at 2246 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal NheI site found at 2071
Illegal NotI site found at 182
Illegal NotI site found at 2225 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal BglII site found at 1070 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal XbaI site found at 1229
Illegal XbaI site found at 2246 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2237
Illegal XbaI site found at 1229
Illegal XbaI site found at 2246
Illegal NgoMIV site found at 105
Illegal NgoMIV site found at 406
Illegal AgeI site found at 1661
Illegal AgeI site found at 1985 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI site found at 346
Illegal SapI site found at 556
Design Notes
N/A - This part was not designed by the UT iGEM team.
Source
This plasmid comes from the Bee Toolkit, as described in: Leonard, S. P., Perutka, J., Powell, J. E., Geng, P., Richhart, D. D., Byrom, M., ... & Barrick, J. E. (2018). Genetic engineering of bee gut microbiome bacteria with a toolkit for modular assembly of broad-host-range plasmids. ACS Synthetic Biology, 7(5), 1279-1290.