Difference between revisions of "Part:BBa K4614002:Experience"
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===Applications of BBa_K4614002=== | ===Applications of BBa_K4614002=== | ||
| + | We constructed an expression vector of R5 and its surface display carrier protein IPN fusion protein, using T7 promoter as the promoter, and induced expression, and after reviewing the literature, we selected to induce 3 h at 37 °C at a final concentration of 1.0 ug/mL in the logarithmic phase, disrupted the bacteria, and performed Western blotting experiments on the supernatant and precipitation of the cell disruption solution to verify the expression of the protein of interest. | ||
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| + | https://static.igem.wiki/teams/4614/wiki/si-parts/jiaotu.jpg | ||
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| + | fig1.Bacterial holoprotein Western blotting development result | ||
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| + | We silicified the mutant strains using the method of silicification of bacteria obtained from the literature, and the control group did the same, we collected the silicified bacteria and observed the bacteria using transmission electron microscopy to obtain the silicification effect of R5 under the silicification conditions we used. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 14:34, 5 October 2023
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Applications of BBa_K4614002
We constructed an expression vector of R5 and its surface display carrier protein IPN fusion protein, using T7 promoter as the promoter, and induced expression, and after reviewing the literature, we selected to induce 3 h at 37 °C at a final concentration of 1.0 ug/mL in the logarithmic phase, disrupted the bacteria, and performed Western blotting experiments on the supernatant and precipitation of the cell disruption solution to verify the expression of the protein of interest.
fig1.Bacterial holoprotein Western blotting development result
We silicified the mutant strains using the method of silicification of bacteria obtained from the literature, and the control group did the same, we collected the silicified bacteria and observed the bacteria using transmission electron microscopy to obtain the silicification effect of R5 under the silicification conditions we used.
User Reviews
UNIQffd1c91beff93e14-partinfo-00000000-QINU UNIQffd1c91beff93e14-partinfo-00000001-QINU