Difference between revisions of "Part:BBa K4656005"

Latest revision as of 13:16, 5 October 2023

PpchA

PpchA, the promoter of pchA, is present in the butyrate sensor derived from EHEC strain O157:H7 discovered by Nakanishi et al. (2009).[1] In the butyrate sensor, PpchA binds to a complex formed by leucine response regulatory protein (Lrp) and butyrate to activate the transcription of pchA regulatory factors.

Reference

[1]. Nakanishi, N., Tashiro, K., Kuhara, S., Hayashi, T., Sugimoto, N., & Tobe, T. (2009). Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia coli. Microbiology (Reading, England), 155(Pt 2), 521–530. https://doi.org/10.1099/mic.0.023499-0

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 143
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 75

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K4656005 short</partinfo>
+<html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/part-pcha.jpg"width="220" height="170"></center></html>
+PpchA, the promoter of pchA, is present in the butyrate sensor derived from EHEC strain O157:H7 discovered by Nakanishi et al. (2009).[1] In the butyrate sensor, PpchA binds to a complex formed by leucine response regulatory protein (Lrp) and butyrate to activate the transcription of pchA regulatory factors.
-PpchA, the promoter of pchA, is present in the butyrate sensor derived from EHEC strain O157:H7, which was discovered by Nakanishi et al. (2009).[1] In the butyrate sensor, PpchA binds to a complex formed by leucine response regulatory protein (Lrp) and butyrate to activate the transcription of pchA regulators.
+===Reference===
+[1]. Nakanishi, N., Tashiro, K., Kuhara, S., Hayashi, T., Sugimoto, N., & Tobe, T. (2009). Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia coli. Microbiology (Reading, England), 155(Pt 2), 521–530. https://doi.org/10.1099/mic.0.023499-0
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+===Sequence and Features===
 <partinfo>BBa_K4656005 SequenceAndFeatures</partinfo>
@@ Line 17: / Line 20: @@
 <partinfo>BBa_K4656005 parameters</partinfo>
 <!-- -->
-===Reference===
-[1]. Nakanishi, N., et al., Regulation of virulence by butyrate sensing in enterohaemorrhagic Escherichia  coli. Microbiology (Reading), 2009. 155(Pt 2): p. 521-530.