Difference between revisions of "Part:BBa K233307:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K233307=== | ===Applications of BBa_K233307=== | ||
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| + | This is essentially an export tag and must be fused upstream of the gene of the protein that is to be secreted out of the cell into the medium. | ||
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| + | The fusion must be in-frame so as to get a functional protein-of-interest. Once this is ensured the tag helps in directing the fusion protein( i.e. of the tag and the protein-of-interest)to the membrane bound Tat. | ||
| + | The Tat (twin-arginine translocation) system delivers folded proteins across biological membranes [[1]] | ||
| + | TorA is the signal sequence that is used in this pathway. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 09:38, 18 October 2009
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K233307
This is essentially an export tag and must be fused upstream of the gene of the protein that is to be secreted out of the cell into the medium.
The fusion must be in-frame so as to get a functional protein-of-interest. Once this is ensured the tag helps in directing the fusion protein( i.e. of the tag and the protein-of-interest)to the membrane bound Tat. The Tat (twin-arginine translocation) system delivers folded proteins across biological membranes 1 TorA is the signal sequence that is used in this pathway.
User Reviews
UNIQ9b6f3695a4ae5dda-partinfo-00000000-QINU UNIQ9b6f3695a4ae5dda-partinfo-00000001-QINU