Difference between revisions of "Part:BBa K4656001"
 
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<partinfo>BBa_K4656001 short</partinfo>
 
<partinfo>BBa_K4656001 short</partinfo>
  
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Tdc is the gene encoding tryptophan decarboxylase (TDC), which can remove the carboxyl group from 5-hydroxytryptophan (5-HTP) and convert it into serotonin (5-hydroxytryptophan, 5-HT) molecules. Tryptophan decarboxylase exists widely in animals and plants, especially in the central nervous system and gastrointestinal tract. Among them, 5-HTP is a precursor substance of neurotransmitter, while serotonin is an important neurotransmitter, similar to intestinal motor in terms of intestinal physiological function, and plays an important role in alleviating chronic constipation.  
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TDC can remove the carboxyl group from 5-hydroxytryptophan (5-HTP) and convert it into serotonin (5-hydroxytryptophan, 5-HT). Tryptophan decarboxylase exists widely in animals and plants, especially in the central nervous system and gastrointestinal tract. 5-HTP is a precursor substance of the neurotransmitter 5-HT, an important neurotransmitter that plays an intestinal motility role in the physiological function of the intestine and plays a vital role in alleviating chronic constipation.
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<html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;cursor: zoom-in;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/tdc-tph.jpg" width="600" height="200"></center></html>
  
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===Usage and Biology===
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<center><b>The synthesis of 5-HT </b></center>
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Our project uses the introduction of Tdc sequences and inducing their expression to produce serotonin to test their efficacy in treating constipation.
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===Usage and Biology===
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In our 5-HT producing circuit, TDC and TPH together catalyze the generation of 5-HT, when sensing a decrease in butyrate concentration. Experimental results about our 5-HT producing circuit can be found in our composite part BBa K4656007.
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<html><BR><BR><center><img style="display: block;-webkit-user-select: none;margin: auto;cursor: zoom-in;background-color: hsl(0,0%,90%);transition: background-color 300ms;" src="https://static.igem.wiki/teams/4656/wiki/part-plam-tph-tdc.jpg" width="600" height="300"></center></html>
  
 
===Sequence and Features===
 
===Sequence and Features===

Latest revision as of 12:28, 5 October 2023


TDC

TDC can remove the carboxyl group from 5-hydroxytryptophan (5-HTP) and convert it into serotonin (5-hydroxytryptophan, 5-HT). Tryptophan decarboxylase exists widely in animals and plants, especially in the central nervous system and gastrointestinal tract. 5-HTP is a precursor substance of the neurotransmitter 5-HT, an important neurotransmitter that plays an intestinal motility role in the physiological function of the intestine and plays a vital role in alleviating chronic constipation.

The synthesis of 5-HT

Usage and Biology

In our 5-HT producing circuit, TDC and TPH together catalyze the generation of 5-HT, when sensing a decrease in butyrate concentration. Experimental results about our 5-HT producing circuit can be found in our composite part BBa K4656007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1248
    Illegal BamHI site found at 732
    Illegal BamHI site found at 834
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 340
    Illegal NgoMIV site found at 775
    Illegal NgoMIV site found at 1330
    Illegal AgeI site found at 478
    Illegal AgeI site found at 1402
  • 1000
    COMPATIBLE WITH RFC[1000]