Difference between revisions of "Part:BBa K4665001"

Revision as of 12:19, 5 October 2023

INPN

This is the N-terminal of ice nucleation protein which will be embedded into the outer cell membrane of E. coli. The sequence coding for the INPN is preceded by a pelB leader sequence. By attaching the pelB signal peptide in front of the INP protein, the fusion protein will be directed towards the bacterial periplasm where it will be anchored in the cell membrane (Singh et al., 2013). The INPN sequence is followed by two front-end sub-repeat sequences important for the stability of the fusion protein (Zhu et al., 2022).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 470
Illegal PstI site found at 592
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 470
Illegal PstI site found at 592
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 470
Illegal PstI site found at 592
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 470
Illegal PstI site found at 592
Illegal NgoMIV site found at 54
Illegal AgeI site found at 555
1000
COMPATIBLE WITH RFC[1000]

Revision as of 12:15, 28 September 2023 (view source) Registry (Talk \| contribs)		Revision as of 12:19, 5 October 2023 (view source) FloorVervuren (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K4665001 short</partinfo>		<partinfo>BBa_K4665001 short</partinfo>

−	This is the N-terminal of ice nucleation protein which will be embedded into the outer cell membrane E. coli. The sequence coding for the INPN is preceded by a pelB leader sequence. By attaching the pelB signal peptide in front of the INP protein, the fusion protein will be directed towards the bacterial periplasm where it will be anchored in the cell membrane (Singh et al., 2013). The INPN sequence is followed by two front-end sub-repeat sequences important for the stability of the fusion protein (Zhu et al., 2022).	+	This is the N-terminal of ice nucleation protein which will be embedded into the outer cell membrane of E. coli. The sequence coding for the INPN is preceded by a pelB leader sequence. By attaching the pelB signal peptide in front of the INP protein, the fusion protein will be directed towards the bacterial periplasm where it will be anchored in the cell membrane (Singh et al., 2013). The INPN sequence is followed by two front-end sub-repeat sequences important for the stability of the fusion protein (Zhu et al., 2022).